Production of monoclonal antibodies

The antigen will be provided by the client and the amount thereof will depend on the nature of the immunogen in question.

In this case, the duration of the development of monoclonal antibodies will extend for a period of time between 4 and 6 months, approximately. The service includes the following modules:

  • Preliminary meetings to discuss the project strategy
  • Immunization of 5 BALB / c mice
  • Hybridization
  • Selection of positive clones by ELISA, or others appropriate immunoassay, followed by two cloning by limit dilution
  • Delivery to the client of the supernatant (approximately 1 ml) of the culture of the positive clones selected for the functional evaluation of the monoclonal antibodies in their own system
  • Growth / Expansion of the hybridoma and cryopreservation
  • Determination of the IgG subclass of hybridomas and mycoplasma test

It will be delivered to the client:

  • Mouse pre-immune serum approx. 500 μl
  • Three cryovials and 15 ml of culture supernatant of each selected hybridoma (maximum three hybridomas)
  • Antibody information