Our services include the cloning into a vector of high copy number, purification and quality control of PCR fragments provided by the client, for use as controls, standards and probes. The basic service consists of the cloning of the PCR fragment into a high copy number vector, check of the construction by PCR and / or restriction analysis, purification and quantification of the plasmid.
Other optional complementary services are:
- Sequencing of the cloned fragments.
- Serial dilutions by quantitative PCR.
- Linearization of the plasmid.