Expression and purification of proteins in yeasts

Expression and Purification in Saccharomyces cerevisiae or Pichia pastoris:

The expression of heterologous proteins in bacterial systems is an effective way to obtain high yields of our desired protein at a low cost. In some cases, yeast systems can be an efficient alternative to express and purify some of these proteins.

The service in both cases includes:

  • Project study
  • Preparation of endotoxin-free plasmid
  • Transformation of yeast strain (s)
  • Evaluation of expression in different conditions (voltage, temperature, time, inductor concentration …)
  • Analysis SDS / PAGE and WB if necessary
  • Purification tests
  • Analysis SDS / PAGE and WB if necessary
  • Expression and purification increased 1L
  • Analysis SDS / PAGE and WB if necessary