Expression and purification in mammals

The working method of the Biomedal services, in expression and purification of proteins in mammals, is divided into several main services:

Cloning in mammalian expression vectors
Small scale expression test (with linear PEI)
Small-scale expression assay (with cationic lipids)
MALDI-TOF mass spectrometry
Review of results by the client (optional)
Half-scale production (with linear PEI)
Half-scale production (with cationic lipids)
Purification of soluble recombinant protein
Purification of proteins fused to insoluble His
Elaboration of detailed final report and shipment of samples