Expression and purification in baculovirus

Expression and purification in Baculovirus offers the advantages of expression in a eukaryotic system with high levels of expression of recombinant genes, as well as easy scaling.

As in the previous cases, the working method of the Biomedal services, in expression and protein purification in Baculovirus, is divided into several main services:

Cloning in a baculovirus expression vector (1 tag)
Cloning into two baculovirus expression vectors (2 different tags)
Generation of recombinant baculovirus (1 tag)
Generation of recombinant baculoviruses (2 different tags)
MALDI-TOF mass spectrometry
Amplification of recombinant baculoviruses for high titer stock
Optimization of the expression
Large-scale production
Purification of soluble recombinant protein
Purification of insoluble His-fused proteins