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Easyeast

  • This cell technology avoids the requirement for chemical, enzymatic or mechanical means to break yeast cell envelopes. The EASYEAST strain series are provided of mutations that allow conditionally fragile cell wall. The main advantage of this system is that intracellular proteins can be released in an easy and efficient way by growth in low osmotic media at moderate temperature (37ºC). High density cultures can also be achieved.

    Advantages:

    • No need for enzymatic or chemical treatment.
    • No need for specialized equipment.

    Applications:

    • Large-scale production of cytoplasmic proteins in yeasts.
    • Heteromultimeric protein production.
    • Bio-assays in yeast for drug discovery.
    • High-throughput protein expression/purification with purification tags.
  • Related Purification kits
    KT-5494 HISTAG 2-Phase Separation System
    Related Purification Reagents
    RS-5495 Lower Solution (HISTAG 2-Phase Separation System) 20 ml
    RS-5496 Top Solution (HISTAG 2-Phase Separation System) 10 ml
    RS-5497 LYT 2-Phase Wash (2x) (HISTAG 2-Phase Separation System) 30 ml
    RS-5498 2x Elution Solution (HISTAG 2-Phase Separation System) 30 ml
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    In case you would like to place an order directly, please, click on the requested products:

    YS-3250 Saccharomyces cerevisiae BY4741 Slant 158 €YS-3251 Saccharomyces cerevisiae EY1 Slant 420 €YS-3252 Saccharomyces cerevisiae EY1-DP Slant 797 €

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