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Reporter Transposons

  • pUT mini-Tn5 System

    The mini-Tn5 are mobile elements able to transpose to the chromosome from a delivery plasmid, by means of the transposase activity encoded by the tnp* gene, which is present in cis in the plasmid but external to the mini-Tn5 element. pUT mini-Tn5 vectors can be used to insert any DNA fragment in the bacterial chromosome, by cloning it within the mini-Tn5 region. Due to the loss of the tnp* gene after insertion, minitransposons are stably integrated into the chromosome and inherited. The different pUTmini-Tn5 vectors can then be used for repeated insertion events and therefore multiple insertions in the same strain are only limited by the availability of distinct selection markers.

    Advantages:

    • Easy and stable insertion of heterologous DNA in the chromosome of the strain of interest, after few simple genetic manipulations.
    • Wide range of inserted fragment sizes.
    • Possibility of multiple insertions in the same strain.
    • Easy modification of the cloning vector.
    • Use of cleavage sites of the rare cutters NotI and SfiI.
    • Wide range of Gram negative recipient strains.

     

    Some of the most common applications of pUTmini-Tn5 vectors are:

    • Cloning.
    • Insertional mutagenesis.
    • Promoter probing.
    • Generation of conditional phenotypes and non-polar mutations.
    • Analysis of transcriptional terminators.
    • Environmental applications: tagging with marker genes for monitoring of released microorganisms and/or transcriptional regulation in the environment.
  • Related Strains
    BS-3233      E. coli DH5a lpir    Stab
    BS-3234      E. coli S17-1 lpir    Stab
    BS-3303      E. coli SM10 lpir    Stab
    BS-3236      E. coli DH5a(pRK2013)    Stab
    BS-3263      E. coli DH5a(pRK2073)    Stab
    KT-4694    E. coli DH5a Competent Cells Set    12 vials of 0.2 ml and pUC18Not
    Related Reagents
    RS-3215    TSS solution – 20 reactions    1.5 ml
    RS-3216    TSS solution – 100 reactions    1.5 ml x 5
    RS-3217    Ampicillin    5 g
    RS-3305    Ampicillin    25g
    RS-3306    Ampicillin    100g
    RS-3218    Chloramphenicol    5 g
    RS-3307    Chloramphenicol    100g
    RS-3315    Erythromycin    5g
    RS-3327    Erythromycin    100g
    RS-3328    Gentamycin    1g
    RS-3329    Gentamycin    5g
    RS-3219    Kanamycin    5 g
    RS-3221    Streptomycin    5 g
    RS-3310    Streptomycin    25g
    RS-3220    Tetracycline    5 g
    RS-3308    Tetracycline    25g
    RS-3309    Tetracycline    100g
    RS-3222    Potassium Tellurite    1 g
    RS-3311    Potassium Tellurite    5g
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    In case you would like to place an order directly, please, click on the requested products:

    CV-3256 pUT/lacZ1 8 µgCV-3257 pUT/lacZ2 8 µgCV-3258 pUT/phoA 8 µgCV-3259 pUT/luxAB 8 µg

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