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pUT mini-Tn5 Vectors

  • The mini-Tn5 are mobile elements able to transpose to the chromosome from a delivery plasmid, by means of the transposase activity encoded by the tnp* gene, which is present in cis in the plasmid but external to the mini-Tn5 element. pUT mini-Tn5 vectors can be used to insert any DNA fragment in the bacterial chromosome, by cloning it within the mini-Tn5 region. Due to the loss of the tnp* gene after insertion, minitransposons are stably integrated into the chromosome and inherited. The different pUTmini-Tn5 vectors can then be used for repeated insertion events and therefore multiple insertions in the same strain are only limited by the availability of distinct selection markers.

    Advantages:

    • Easy and stable insertion of heterologous DNA in the chromosome of the strain of interest, after few simple genetic manipulations.
    • Wide range of inserted fragment sizes.
    • Possibility of multiple insertions in the same strain.
    • Easy modification of the cloning vector.
    • Use of cleavage sites of the rare cutters NotI and SfiI.
    • Wide range of Gram negative recipient strains.

     

    Some of the most common applications of pUTmini-Tn5 vectors are:

    • Cloning.
    • Insertional mutagenesis.
    • Promoter probing.
    • Generation of conditional phenotypes and non-polar mutations.
    • Analysis of transcriptional terminators.
    • Environmental applications: tagging with marker genes for monitoring of released microorganisms and/or transcriptional regulation in the environment.
  • pUT mini-Tn5 Cm kit

    • pUT mini-Tn5 Cm, 2 μg.
    • E. coli DH5a lpir, stab.
    • E. coli S17-1 lpir, stab.
    • E. coli DH5a(pRK2013), stab.
    • TSS solution, 1.5ml.

     

    pUT mini-Tn Km kit

    • pUT mini-Tn5 Km, 2 μg.
    • E. coli DH5a lpir, stab.
    • E. coli S17-1 lpir, stab.
    • E. coli DH5a(pRK2073), stab.
    • TSS solution, 1.5ml.

     

    pUT mini-Tn Sm/Sp kit

    • pUT mini-Tn5 Sm/Sp, 2 μg.
    • E. coli DH5a lpir, stab.
    • E. coli S17-1 lpir, stab.
    • E. coli DH5a(pRK2013), stab.
    • TSS solution, 1.5ml.

     

    pUT mini-Tn Tc kit

    • pUT mini-Tn5 Tc, 2 μg.
    • E. coli DH5a lpir, stab.
    • E. coli S17-1 lpir, stab.
    • E. coli DH5a(pRK2013), stab.
    • TSS solution, 1.5ml.

     

    PANC-1 mini-Tn5 package

    • pUT mini-Tn5 Km, 2 μg.
    • pUT mini-Tn5 Cm, 2 μg.
    • pUT mini-Tn5 Sm/Sp, 2 μg.
    • pUT mini-Tn5 Tc, 2 μg.

     

    PANC-1plus miniTn5 package

    • pUT mini-Tn5 Km, 2 μg.
    • pUT mini-Tn5 Cm, 2 μg.
    • pUT mini-Tn5 Sm/Sp, 2 μg.
    • pUT mini-Tn5 Tc, 2 μg.
    • E. coli DH5a lpir, stab.
    • E. coli S17-1 lpir, stab.
    • E. coli DH5a(pRK2013), stab.
    • E. coli DH5a(pRK2073), stab.
    • TSS solution, 1.5ml.

     

    pUT mini-Tn5Tel kit

    • pUT mini-Tn5Tel vector, 2 μg.
    • Potassium Tellurite, 100mg.
    • E. coli DH5a lpir, stab.
    • E. coli S17-1 lpir, stab.
    • E. coli DH5a(pRK2013), stab.
    • TSS solution, 1.5ml.

     

  • Related Strains
    BS-3233      E. coli DH5a lpir    Stab
    BS-3234      E. coli S17-1 lpir    Stab
    BS-3236      E. coli DH5a(pRK2013)    Stab
    BS-3263      E. coli DH5a(pRK2073)    Stab
    KT-4694    E. coli DH5a Competent Cells Set    12 vials of 0.2 ml and pUC18Not
    Related Reagents
    RS-3215    TSS solution – 20 reactions    1.5 ml
    RS-3216    TSS solution – 100 reactions    1.5 ml x 5
    RS-3445    Potassium Tellurite    100mg
    RS-3222    Potassium Tellurite    1g
    RS-3311    Potassium Tellurite    5g
    RS-3217    Ampicillin    5 g
    RS-3305    Ampicillin    25g
    RS-3306    Ampicillin    100g
    RS-3218    Chloramphenicol    5 g
    RS-3307    Chloramphenicol    100g
    RS-3315    Erythromycin    5g
    RS-3327    Erythromycin    100g
    RS-3328    Gentamycin    1g
    RS-3329    Gentamycin    5g
    RS-3219    Kanamycin    5 g
    RS-3221    Streptomycin    5 g
    RS-3310    Streptomycin    25g
    RS-3220    Tetracycline    5 g
    RS-3308    Tetracycline    25g
    RS-3309    Tetracycline    100g
    RS-3222    Potassium Tellurite    1 g
    RS-3311    Potassium Tellurite    5g
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    In case you would like to place an order directly, please, click on the requested products:
    CV-3223 pUT mini-Tn5Cm vector 2µgCV-3224 pUT mini-Tn5Km vector 2µgCV-3225 pUT mini-Tn5Sm/Sp vector 2µgCV-3226 pUT mini-Tn5Tc vector 2µgKT-3228 pUT mini-Tn5Cm kit 1 kitKT-3229 pUT mini-Tn5Km kit 1 kitKT-3230 pUT mini- Tn 5Sm/Sp kit 1 kitKT-3231 pUT mini-Tn5Tc Kit 1 kitKT-3260 PANC-1 mini-Tn5 package 1 kitKT-3261 PANC-1plus miniTn5 package 1 kitCV-3227 pUT mini-Tn5Tel vector 2µgKT-3232 pUT mini-Tn5Tel kit 1 kit

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