Biomedal offers all the necessary reagents for its own developed purification technologies such as LYTAG Expression and Purification Systems, LYTAG Two Phase Purification System and Histag Two Phase Purification Systems.
LYTAG system allows the single-step purification of proteins fusion to LYTAG using LYTRAP resin, a simple and efficient chromatographic support. Binding conditions are gentle and do not involve covalent modifications, in such a way that the fusion protein is highly stable once bound to the resin.
Choline Chloride solution
LYTAG fusions protein are selectively eluted using choline-containing buffers.
Salicylate solution is used as the inducer of many protein expression systems, including Biomedal’s pALEX2 a,b,c systems.
Prolyse Protein Extraction solution
Prolyse Protein Extraction solution Is an amine-free solutiuon developed for LYTAG protein purification technology. It allows bacterial wall disruption without pressure or sonication requirements. Proteins are released and obtained in active soluble form.
LYTAG Two Phase Purification System solutions
LYTAG Two-Phase Purification system is a protein purification system based on the use of two aqueous components. The method relies on the affinity of the protein tag LYTAG for one of the two-phase components, allowing recombinant protein separation and purification from cellular extracts or culture media. In the procedure, the LYTAG-fused protein is retained in one of the aqueous phases while most of the undesired proteins can be removed by simply discarding the opposite phase. After replenishing the system with fresh phase, the protein of interest can be easily recovered in it, with high purity, by reversing its localization with the addition of choline, the specific LYTAG ligand.